hyalograft_fibroblasts_aStemDerm is Cytoprosthetic Hybrid that is composed of stem cells cultured on a thin membrane, a special scaffold, composed of an esterified  form of hyaluronic acid. Stem cells can be differentiated into a number of cells, such as skin fibroblasts and keratinocytes. They also give rise to vascular endothelial cells that are essential for the viability for the regenerated skin. Stem cells have the ability to produce a number of growth factors and cytokines, that activate the migration of other fibroblasts, epithelial cells and vascular endothelial cells at the site of the injury, activating this way the internal regeneration mechanism of the skin. For these reasons, the presence of stem cells is very important for the regeneration of damaged skin and therefore the treatment of wounds, ulcers and burns.

The 3-D culture of stem cells on a hyaluronic acid scaffold activates their growth,  so that they start forming the skin layers. When applied at the site of the injury, this construct will be incorporated to the skin, promoting this way the closure of the wound, through the formation of new skin layers.

In order to prepare StemDerm, a small punch biopsy (5mm) is collected from the abdominal area of the patient. This punch biopsy is subsequently placed into a special tube containing a buffered medium and the sample is transferred to our laboratories. Upon arrival to our premises, the sample is processed for the isolation of adipose tissue (on average, an amount of 0,13g of fat is isolated per biopsy). Following a careful micromanipilation of the adipose tissue, stem cells are isolated and tested for their quality and possible infections. They are subsequently expanded in special incubators and culture media.

After approximately 4 weeks, when the appropriate number of stem cells is reached, the cells are cultured on a 3-D membrane (scaffold) for an additional 8 days for the formation of the StemDerm. The Cytoprosthetic Hybrid is then sent to the hospital for its application on the wound, burn or ulcer.

Direct application of the StemDerm on the wound, leads to:

  • Full regeneration of the skin layers
  • Revascularization
  • Migration of additional keratinocytes & fibroblasts
  • Re-epithelization of the skin